slowfade gold antifade mountant with dapi s36938  (Corning Life Sciences)

Journal: bioRxiv

Article Title: DNA O-MAP uncovers the molecular neighborhoods associated with specific genomic loci

doi: 10.1101/2024.07.24.604987

Figure Lengend Snippet: A) Schematic of DNA O-MAP. B) Overview of telomere targeted DNA O-MAP experiment. C) Fluorescent microscopy data showing the observed patterns of DNA (DAPI, left) and in situ biotinylation detected by staining with fluorescent streptavidin conjugates (middle, left)l. D) Significant gene sets identified by the Gene Set Enrichment Analysis of the proteins enriched by the telomere probe. E) DNA O-MAP telomeric proteins mapped onto the BioPlex interaction network , . The red box highlights shelterin complex proteins. Nodes are colored by the fold-enrichment compared to a no-primary-probe control shown in C, excluding unconnected nodes. F) Telomeric proteins observed in five previous datasets (PICh, C-BERST, CAPLOCUS, CAPTURE, BioID) superimposed onto , colored by the number of prior datasets where the protein was present and including unconnected nodes. Scale bars, 5 µm.

Article Snippet: Washed cells were immobilized on glass slides with Slowfade Gold Antifade Mountant with DAPI (Thermo Fisher S36938) and coverslips for confocal imaging of FISH signal.

Techniques: Microscopy, In Situ, Staining, Control

Journal: Molecular Biology of the Cell

Article Title: Rab21 in enterocytes participates in intestinal epithelium maintenance

doi: 10.1091/mbc.E21-03-0139

Figure Lengend Snippet: 3D reconstruction of a control intestine stained with DAPI and Phalloidin-Alexa546 . Images were acquired on a Z1 LightSheet microscope (Zeiss), using a 20x Plan-APOCHROMAT/1NA objective and a volume of approximately 25 μm was imaged every 0.5 μm. The movie was generated using Imaris and depicts surfaces.

Article Snippet: Tissues were mounted in SlowFade Gold Antifade Mountant with DAPI (#S36938; Thermo Fisher Scientific).

Techniques:

Journal: Molecular Biology of the Cell

Article Title: Rab21 in enterocytes participates in intestinal epithelium maintenance

doi: 10.1091/mbc.E21-03-0139

Figure Lengend Snippet: 3D reconstruction of an intestine depleted of Rab21 in enterocytes stained with DAPI and Phalloiding-Alexa546 . Images were acquired on a Z1 LightSheet microscope (Zeiss), using a 20x Plan-APOCHROMAT/1NA objective and a volume of approximately 25 μm was imaged every 0.5 μm. The movie was generated using Imaris and depicts surfaces.

Article Snippet: Tissues were mounted in SlowFade Gold Antifade Mountant with DAPI (#S36938; Thermo Fisher Scientific).

Techniques:

Journal: Molecular Biology of the Cell

Article Title: Rab21 in enterocytes participates in intestinal epithelium maintenance

doi: 10.1091/mbc.E21-03-0139

Figure Lengend Snippet: Rab21 expression in enterocytes is required to maintain correct intestinal epithelium morphology. (A) Schematic of Drosophila midgut compartmentalization. (B–D) Intestine of a Rab21-GAL4>UAS-GFP:Rab21 WT transgenic fly. (B) Arrowheads indicate the copper cell region and the distal posterior midgut and (C) Rab21 enrichment in enterocytes. (D) Arrows show Rab21 enrichment in other Armadillo-positive (Arm + ) cell types. Images are representative of n = 15 guts. (D′, D′′) Magnifications of the areas highlighted by arrows in D. (E) In adult flies, expression of two independent Rab21 RNAi hairpins or Rab21 DN in enterocytes was achieved using Myo1A ts -GAL4. (F) Inverted binary images of Drosophila guts stained with DAPI and quantification of cell density. (G) Immunostaining for Coracle (Cora). Images are representative of n = 15–20 guts. (H) Epithelial organization of guts stained with DAPI and phalloidin. The percentages of guts with single- and multilayered epithelia are shown ( n = 14 guts). (I) Transverse views of guts stained with DAPI (blue) and phalloidin (red; to detect F-actin) with measurements of luminal areas. (J) Survival curves in controls and flies expressing Rab21 RNAi or Rab21 DN . For the LacZ, Rab21 RNAi-1, and Rab21 RNAi-2 conditions , n = 46, 75, and 47 flies, respectively. For Rab21 DN n = 30 flies. For all experiments N = 3, except for I and Rab21 DN in J, where N = 2. **** P < 0.0001 by one-way ANOVA followed by Dunnett’s test (F).

Article Snippet: Tissues were mounted in SlowFade Gold Antifade Mountant with DAPI (#S36938; Thermo Fisher Scientific).

Techniques: Expressing, Transgenic Assay, Staining, Immunostaining